Roles of Two F-Box Proteins: FBXL14 in the Periosteum and FBXW2 at Elastic Fibers

I previously reported that F-box/leucine-rich repeat protein 14 (FBXL14) expressed in periosteum-derived cells, and F-box WD-40 domain-containing 2 (FBXW2) the periosteum form a fiber-like structure. Here, two culture medium conditions, is, media with without ascorbic acid, were compared during explant culture. In absence of expression patterns osteocalcin, FBXW2, elastin using fluorescent immunostaining weeks 3–5. By observing periosteum, cambium layer bone, demonstrated FBXL14 micro-vessels bone lacuna. Fluorescent revealed that, was thin. Conversely, presence formed thick membrane-like structure inside multilayer cells (PDCs) strong. The osteocalcin FBXW2 similar. Elastin retained its fiber for up to five weeks. Although regions similar elastin, they could not retain their structures. conclusion, appears play role preparing native scaffold forming multilayered sheet PDCs periosteum. appear separate from elastic fibers calcification.